International Journal of Fisheries and Aquatic Research

International Journal of Fisheries and Aquatic Research


ISSN: 2456-7248

Vol. 2, Issue 2 (2017)

Extraction, identification of bioactive compounds and in vitro antioxidant activity potential in freshwater ampullariidae snail Pila virens

Author(s): M Gayathri, M Ramasamy, N Santhiya
Abstract: Objective:Molluscs are considered as a significant natural source to derive many narrative biological active compounds. Animal extract are usually complex mixtures of bioactive molecules, including proteins and peptides. Freshwater gastropods are found to be a vital source of valuable bioactive substances. These bioactive compounds are involved in various biological functions such as communication, infection, reproduction and self- defense. Discovery of freshwater gastropods bioactive potential and their curious roles are still limited. Experimental approach:Consequently, the present study was evaluated on the bioactive compound extraction; identification and In-vitro antioxidant activity were gritty by total antioxidant activity, DPPH (diphenylpicrylhydrazyl) assay, hydroxyl radical scavenging activity, superoxide radical scavenging, reducing power and ferrous ion chelating from active fraction of Pila virens tissue methanolic extract at various concentrations (20-250μg/ml). Findings:The results of freshwater gastropod methanol tissue extracts exhibited significant total antioxidant activity, DPPH (diphenylpicrylhydrazyl) assay, hydroxyl scavenging and superoxide scavenging activity which predicted as 67.09%, 74.83%, 60.21% and 59.89%. Conclusion:These results concluded that, the tissue extract of Pila virens has novel bioactive compound, antioxidant potential and it has to further characterize to improve the pharmacological active freshwater natural products.
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