Sub lethal effects of ammonia at different levels of ph on chosen physiological and biochemical parameters in Labeo rohita (Hamilton, 1822)
L Roselin Rajathi, R Niranjani, R James
Effects of ammonia toxicity at different levels of pH on growth, organic reserves, respiratory enzyme activities and blood parameters were studied in L. rohita for 30 days. The rate and efficiency of conversion or growth were high in NH3 + pH7 group followed by control and NH3 + pH8 groups. However, other exposures of NH3 + pH 6 and 9 showed a drastic and negative reduction in the rate and efficiency of conversion in L. rohita. The FCR value observed in NH3 + pH7 and control groups was low as compared to other groups. The tested proximate compositions were also significantly (P < 0.05) reduced in fish exposed to NH3 alone and NH3 with acidic (pH6) and alkaline medium (pH9) as compared to other exposures. An oxidative enzyme, succinate dehydrogenase (SDH) activity and the blood parameters, RBC count, Hb count, Ht, MCV, MCH and MCHC values were high in control and NH3 + pH7 groups and it significantly (P<0.05) declined in fish exposed to ammonia with increasing and decreasing of pH levels. The glycolytic anaerobic enzyme, glyceraldehyde dehydrogenase (GDH) and WBC count and ESR values showed the reverse trend against SDH activity.