Surveillance and quantitative analyses of common gut bacteria in freshwater prawn Macrobrachium rosenbergii fed with Spirulina platensis, Chlorella vulgaris and Azolla pinnata incorporated feed
Radhakrishnan S, Saravana Bhavan P, Muralisankar T
The present study was conducted to assess the surveillance of gut bacterial flora in formulated feed fed Macrobrachium rosenbergii postlarvae. The feed was prepared by 50% fishmeal replaced with Spirulina platensis, Chlorella vulgaris and Azolla pinnata and these were served as experimental feed. The fishmeal feed served as control. The experiment was prolonged for 90 days. The final day of experiment, the prawns were subjected to analyses the surveillance of bacterial flora in gut environment system. The culture water contain bacterial flora also identified. There were six genera of common gut microflora, Bacillus sp. Pseudomonas sp. Escherichia coli, Streptococcus sp. Proteus sp. Salmonella sp. were identified in culture water and feed fedprawn group, whereas the Salmonella sp. was not detected in experimental feed fed prawn groups. There were six genus of common gut microflora (Bacillus sp. Pseudomonas sp. Escherichia coli, Streptococcus sp. Proteus sp. Salmonella sp.) were identified in control feed fed PL, whereas the Salmonella sp. was not identified in experimental feed fed PL groups. The present results revealed that, the pathogenic bacteria, Salmonella sp. colony could have been eradicated by feeding C. vulgaris, S. platensis and A. pinnata incorporated feed. The present result suggested that S. platensis. C. vulgaris and A. pinnata can be used as alternative feed ingredients for maintain the healthy gut micro flora system of Macrobrachium rosenbergii postlarvae.