Culture of Chlorella vulgaris in press mud media as sugar mill waste
ANMAI Khan, MAB Habib, MS Hossain, MI Miah
Chlorella vulgaris was cultured in different concentrations of press mud medium (PMM0.5 g/l, PMM1.0 g/l, PMM1.5 g/l and PMM2.0 g/l) and in Bold basal medium (BBM) as control. Maximum cell number of Chlorella vulgaris was recorded on 8th day in different treatment where PMM1.0 g/l obtained the highest rank and PMM2.0 g/l obtained the lowest. Specific growth rate (SGR) and total biomass of cells indicate that maximum Chlorella vulgaris were grown in PMM1.0 g/l. The maximum SGR of Chlorella vulgaris was determined 0.55 in PMM1.0 g/l followed by 0.53 in BBM, 0.52 in PMM0.5 g/l, 0.46 in PMM1.5 g/l, and 0.43 in PMM2.0 g/l which were supported by cell number, total biomass and chlorophyll a content while optical density showed slightly higher in BBM may be due to cell nutrition and media turbidity. Maximum protein was found of Chlorella vulgaris grown in PMM1.0 g/l followed by that grown in BBM, and then other concentrations of PMM. Lipid content of Chlorella vulgaris grown in all the concentrations of PMM were found significantly (P<0.01) higher than that of BBM.