Vol. 3, Issue 2 (2018)
Culture of Chlorella vulgaris in press mud media as sugar mill waste
Author(s): ANMAI Khan, MAB Habib, MS Hossain, MI Miah
Abstract: Chlorella vulgaris was cultured in different concentrations of press mud medium (PMM0.5 g/l, PMM1.0 g/l, PMM1.5 g/l and PMM2.0 g/l) and in Bold basal medium (BBM) as control. Maximum cell number of Chlorella vulgaris was recorded on 8th day in different treatment where PMM1.0 g/l obtained the highest rank and PMM2.0 g/l obtained the lowest. Specific growth rate (SGR) and total biomass of cells indicate that maximum Chlorella vulgaris were grown in PMM1.0 g/l. The maximum SGR of Chlorella vulgaris was determined 0.55 in PMM1.0 g/l followed by 0.53 in BBM, 0.52 in PMM0.5 g/l, 0.46 in PMM1.5 g/l, and 0.43 in PMM2.0 g/l which were supported by cell number, total biomass and chlorophyll a content while optical density showed slightly higher in BBM may be due to cell nutrition and media turbidity. Maximum protein was found of Chlorella vulgaris grown in PMM1.0 g/l followed by that grown in BBM, and then other concentrations of PMM. Lipid content of Chlorella vulgaris grown in all the concentrations of PMM were found significantly (P<0.01) higher than that of BBM.
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