Vol. 5, Issue 1 (2020)
Advanced studies on diagnosis of pseudomonas septicemia in some marine fishes
Author(s): Eissa IAM, Takwa Ismail, Shimaa Mansour
Abstract: A total number of two hundreds (200) naturally infected and freshly caught marine fishes divided as 100 seabass (Dicentrarchus labrax) and 100 Tilapia zillii were collected randomly and seasonally from Lake Temsah in Ismailia governorate. Fishes subjected to clinical, postmortem and bacteriological studies to isolate and identify the bacteria causing the disease. The most isolated bacteria were Pseudomonas aeruginosa which identified by using traditional methods and Vitek 2 system. The identification was confirmed by detection of 16s rDNA gene and two virulence genes (toxA and oprL) of pathogenic Pseudomonas aeruginosa using PCR. The most prevalent and virulent bacteria isolated from naturally infected marine fishes was Pseudomonas aeruginosa. The experimentally infected Tilapia zillii by intraperitoneal route (I/P) with Pseudomonas aeruginosa showed the same signs recorded in the naturally infected marine fishes. The Mortality and morbidity rates were monitored daily for one week.
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