Production of Single cell protein (SCP) from Saccharomyces cerevisiae was investigated. Baker yeast, Saf-Instant S. cerevisiae was activated on Peptone Yeast Glucose (PYG) media. Agro industrial waste, cane molasses and brewery waste were used as substrate to produce SCP biomass for fish feed. For optimization, basal media was used for S. cerevisiae culture. Optimization was done to find the effectiveness of cane molasses, ammonium source and brewery waste on the growth of the S. cerevisiae. The optimization results showed that basal media with 60 brix 10 % molasses, 2g/l-(NH₄)₂ SO₄, 5 % brewery waste on 4^th day of fermentation period. Under the optimum parameter, batch fermentation process was done for SCP production, the results revealed that maximum protein content for dry biomass was 29 % after 4^th day of fermentation period.