Fish processing waste could be regarded as processing the renewable biomass resource for bio-refinery. Hydrolysis of fish waste through natural cold fermentation is aimed primarily at industrial application. Fish hydrolysate generally shows a beneficial effect on growth performances and feed utilization at low inclusion levels. The performance is postulated to be due to balance of free amino acids, fatty acids, macro and micro nutrients which are digested, absorbed and utilized. Wet fish waste is acidified with molasses, fermentation causes hydrolysis of fish waste is called “Fish Hydrolysate”.