International Journal of Fisheries and Aquatic Research

Authors
Ting Lu, Nilogetha Rajasinn

Abstract
An odd phenomenon was observed in an experiment of microinjecting plant extracted chloroplasts to AB wildtype zebrafish (Danio rerio) spawn. Chloroplasts was extracted from spinach (Spinacia oleracea Linn.) (provided by Southwest University, China) (density information not provided by Southwest University, China). Extracted chloroplasts were diluted to a level that won’t cause spawn lethal and then microinjected into the yolk of spawn. The chloroplasts were RFP labeled for later confirmation of existence in zebrafish spawn and larvae. The microinjected spawn was then hatched and observed in PTU (propylenethiourea) dealed fresh water so the pigment won’t sedate (the zebrafish will be kept transparent). The spawn and hatched larvae later were kept fasting condition. The spawn and hatched larvae were kept in a condition of 14-hour light and 10-hour darkness and 28 ℃ fresh water every day. The hatched larvae of microinjected chloroplasts were observed in number increase (no mortal found after hatching even under fasting condition). Assumption of possible fission of chloroplasts microinjected zebrafish larvae was therefore made. The experiment was repeated three times by Ting Lu and each time the phenomenon was confirmed.