This study aims to investigate the effects of four diets with different energy level; 3000, 3100, 3200 and 3300 Kcal/kg, on testes biochemical characteristics ofClarias jaensis. 360 male and female (97 ± 9 g) were divided into four groups, and fed with diets during 5 months. 12 males were sacrificed per group at the end to determine the biochemical characteristics of their testes. The high value of body weight (263.00 ± 29.70 g) and total length (32.63 ± 0.85 cm) were recorded at 3100 Kcal/kg (P<0.05). Testes weight were significantly higher at 3000 Kcal/kg. Except the total cholesterol (8.91 ± 1.11 mg/dl) and LDL (2.31 ± 0.01 mg/dl), other biochemical characteristics of testes were significantly higher at 3000 Kcal/kg and the low values were recorded with other energy level. Therefore; 3000 Kcal/kg was found to be the optimal energy level for a good quality of Clarias jaensis testes.