A study was conducted to monitor the quantitative bacterial flora in the Glossogobius giuris, water and sediment of the pond. Mean water temperature (°C), dissolve oxygen (mg/l), and pH were 19±3, 30±4, 5.6±0.5, 5.1±0.2, 6.8±0.4 and 7.1±0.3 in winter and summer respectively. In winter total viable bacterial count in water ranged from 9.15×10³ to 6.20×10⁴ CFU/ml and in sediment ranged from 7.72×10⁷ to 3.80×10⁸ CFU/g. In summer, it ranged from 7.74×10⁴ to 4.67×10⁵ CFU/ml in water and 4.84×10⁸ to 2.77×10⁹ CFU/g in sediment. On the other hand, in fish, total viable plate count (TVC) ranged from 8.17×10⁵ to 1.12×10⁶ CFU/g in winter and 4.60×10⁶ to 3.06×10⁷ CFU/g in summer from the pond. Gram-negative rods (77%) and motile bacteria (78%) were dominated. Present study on the bacteriology of the pond was very useful in gathering a lot of valuable information regarding the physico-chemical parameters of water and the bacterial load of water, sediment and fish samples.