Fisheries resource of Rajshahi and Khulna Divisions of Bangladesh was assessed and a taxonomic account of biodiversity has been presented. The taxonomic account of fish biodiversity of the study area comprises 160 species, 97 genera, 44 families, 12 orders. There were 130 species of fishes found in Rajshahi division and 126 species in Khulna division. Ninety six species are common in both divisions. The endemic species of Rajshahi division is 34 and Khulna division is 30 species. The fish fauna listed from Study area only one class Osteichthyes. It belong to the orders of clupeiformes, cypriniformes, siluriformes, angulliformes, symbranchiformes, beloniformes, cyprinodontiformes, perciformes, channiformes, tetraodontiformes, mugiliformes and mastacembeliformes.