The feeding profile of Labeo rohita in Belan River in India was studied to understand their feeding habits and dietary preferences. In order to the study of food and feeding habit of Indian Major Carp, Labeo rohita samples were collected from the commercial catches during 2021 December to February 2022 at two different sites of Belan River at Prayagraj, Uttar Pradesh. During this sampling process, I have collected 247 samples of Labeo rohita in which 124 are male and 123 are female. The analysis of gut content of the Labeo rohita revealed that phytoplankton formed main food item, next important food item is plant items and remaining of unidentified items. It was also seen that there were considerable variations in the percentage of different food items in the gut of fishes. The results showed that Labeo rohita are herbivorous. However, Labeo rohita had a higher preference for plant-based food items. The study also highlighted the importance of environmental factors such as water temperature and flow rate in determining the feeding habits of these fish.